Accession Number:

AD1039459

Title:

Role of Ca ++ Influx via Epidermal TRP Ion Channels

Descriptive Note:

Technical Report,15 Sep 2015,14 Sep 2016

Corporate Author:

Duke University Durham United States

Personal Author(s):

• Liedtke,Wolfgang

Report Date:

2016-10-01

Pagination or Media Count:

44.0

Abstract:

To benefit military veterans with amputations who suffer skin problems on their amputation stumps, this proposal describes mechanistic studies to pave the way for novel methods of improving skin barrier function at the residual limb-prosthetic interface. Signaling systems in skin will be modulated to increase barrier function, attenuate irritant dermatitis, and characterize the underlying signaling mechanisms so that they can become better targets for treatment. Progress in year 2 of the funding period is described in this Annual Progress Report. We maintained all the necessary regulatory approvals from the Durham VA, Duke University IRB and the DoD to conduct the human experimentation. We set up experiments in primary skin cells for mechanical stress, which we found disrupts skin barrier function. We also found that activation of ion channel TRPV4 can re-normalize barrier function of the skin that has been disrupted by mechanical stress. We also found this particular pattern for keratinocytes regulatory volume decrease, as a surrogate of their capability to moisturize.

Descriptors:

• amputations
• military medicine
• inflammation
• epithelial cells
• therapeutics
• proteins
• peptides
• neurons
• biological factors
• membrane potentials
• mass spectrometry
• neuroglia
• residual limbs
• peripheral nervous system
• pain
• skin diseases
• stress (physiology)
• epidermis

Subject Categories:

• Stress Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE