MASS SPECTROMETRY PROTEOMICS METHOD AS A RAPID SCREENING TOOL FOR BACTERIAL CONTAMINATION OF FOOD
Technical Report,01 Mar 2010,31 Dec 2011
ARMY EDGEWOOD CHEMICAL BIOLOGICAL CENTER APG MD ABERDEEN PROVING GROUND United States
Pagination or Media Count:
Every year in the United States there are over 48 million cases of foodborne illness. Traditional microbiological techniques require multiple enrichments using selective media for pathogen detection. Accurate identification of the offending pathogen is necessary to provide the most appropriate outbreak response and patient care. The mass spectrometry proteomics method MSPM does not require enrichment and is not affected by pathogens. The ability to use the MSPM to correctly classify whether or not food samples were contaminated with Salmonella enterica serotype Newport in this blinded pilot study resulted in a high level of sensitivity and specificity 99 and 98.6 , respectively. The study involved mashed potato samples spiked at a concentration of 106 cfumL. These initial studies are encouraging and require further evaluation in more complex food matrices and at various pathogen concentrations to validate MSPM as a useful foodborne pathogen diagnostic tool.
- Miscellaneous Detection and Detectors