SIGNALING PATHWAYS ASSOCIATED WITH VX EXPOSURE IN MESENCHYMAL STEM CELLS
Technical Report,01 Mar 2016,31 Oct 2016
EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND United States
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Mesenchymal stem cells MSCs are multipotent adult stem cells that are key regulators of tissue maintenance and repair. These cells have been identified in several different tissues, but they typically originate in the bone marrow and then circulate through the body in the bloodstream. The U.S. Army Edgewood Chemical Biological Center BioDefense Branch members and academic laboratories have demonstrated that bone marrow-derived MSCs exposed to organophosphate OP pesticides sustain significant changes in their ability to proliferate and differentiate. In the literature, OP compounds were shown to affect the activity and expression levels of acetylcholinesterase AChE however, it is still unclear whether the effect on AChE is caused by these changes. It is also possible that these OP compounds have other unknown secondary effects on MSCs. In this study, we exposed MSCs to the OP chemical warfare agent 145O-ethyl S-2-diisopropylaminoethyl methylphosphonothioate VX and performed phospho-array and phosphoproteomic analysis to gain insights into the signaling pathways associated with VX exposure.
- Anatomy and Physiology
- Chemical, Biological and Radiological Warfare