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Accession Number:
AD1038439
Title:
DARPA ANTIBODY TECHNOLOGY PROGRAM STANDARDIZED TEST BED FOR ANTIBODY CHARACTERIZATION: CHARACTERIZATION OF TWO MS2 SCFV ANTIBODIES PRODUCED BY THE UNIVERSITY OF TEXAS
Descriptive Note:
Technical Report,01 Oct 2010,01 Sep 2012
Corporate Author:
EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND United States
Report Date:
2017-05-01
Pagination or Media Count:
32.0
Abstract:
Antibody selection for inclusion in a final assay format has relied on the performance of an antibody in an enzyme-linked immune sorbent assay with little regard to the quantification of the full spectrum of variables affecting antibodyantigen interactions. The Joint Product Management Office for Bio surveillance JPMO BSV, Critical Reagents Program has instituted a quality program for the standardization of test methods to support comprehensive characterization and comparison of the physical and functional properties of antibody reagents within its repository. The development and standardization of antibodytesting provides JPMO BSV with an invaluable platform for the provision of consistent, high-quality assays and reagents for current bio detection platforms and for the development and validation of future systems. This platform will be used to characterize and evaluate the MS2 recombinant antibody produced and supplied by the Defense Advanced Research ProjectAgency-funded investigator, the University of Texas Austin, TX, for affinity and stability enhancements. The results of this study provide standardized parametric data on antibody properties and performance. They contribute to the development of a decisional analysis tool for expanding the confidence level of antibody-based reagent selection to optimize field operational and performance metrics for future detection and diagnostic platforms.
Distribution Statement:
APPROVED FOR PUBLIC RELEASE
