Accession Number:

AD1034662

Title:

Multifunctional PSCA Antibody Fragments for PET and Optical Prostate Cancer Imaging

Descriptive Note:

Technical Report,30 Sep 2015,29 Sep 2016

Corporate Author:

University of California, Los Angeles Los Angeles United States

Personal Author(s):

Report Date:

2016-10-01

Pagination or Media Count:

10.0

Abstract:

The goals of this initial year of funding were to develop engineered antibody fragments cys-diabodies and cys-minibodies suitable for labeling with positron-emitting radionuclides and fluorescent dyes for multimodal imaging of prostate cancer. A novel cys-minibody recognizing PSCA prostate stem cell antigen has been produced. Conjugation conditions have been developed for radiolabeling with either and I-124 and Zr-89 via lysine random or cysteine site-specific residues of the proteins. Targeting, imaging, and biodistribution studies in mouse models of prostate cancer confirm high activity in PSCA-positive tumors with excellent visualization by PET imaging. Recently, dual-labeling with radionuclide and fluorescent dye of the cys-minibody has been developed and evaluated in mouse models.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE