Super-Resolution Microscopy by Microspheres Embedded in Movable Slabs
Journal Article - Open Access
MIT Lincoln Laboratory Lexington United States
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Recent years have witnessed a breakthrough in achieving truly nanoscale resolution by a fluorescent super-resolution microscopy. The label-free microscopy develops more slowly than fluorescent microscopy for two reasons. First, the mechanisms of label-free imaging rely on much more subtle light-scattering processes in nanoplasmonic or biomedical objects that result in lower effective contrast of images. Second, good point-sources in a form of dye-molecules, fluorophores or quantum dots are not readily available in a label-free microscopy that complicates the experimental quantification of resolution.