Activation of Central Pattern Generator for Respiration Following Complete High Cervical Spinal Cord Interruption
Technical Report,01 Sep 2015,31 Aug 2016
DREXEL UNIVERSITY PHILADELPHIA United States
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The original hypothesis was that intraspinal inhibitory circuits GABA- and Glycinergic play an important role of in the control of a spinal central pattern generator CPG for breathing. These inhibitory spinal interneurons were hypothesized to contribute to suppression of the respiratory CPG in both intact and post-injury high cervical transection conditions. Adhering to the experiments outlined in our SOW, spinal respiratory neurons cervical C3-C5 and C1-C2 levels were characterized by their location, pattern via extra- and intracellular recordings and sensitivity to blockers of GABAa and Glycine receptors GABAzine and strychnine.CPG-specific inspiratory bursts, recorded from phrenic nerves, were observed after application of GABAzine and strychnine over C3-C5 cervical segments in spinally transected and intact animals Ghali and Marchenko, 2016. These spinal bursts were not phase-locked to the supraspinal ponto-medullary respiratory rhythm. We recorded spinal interneurons related to the spinal respiratory CPG, a promising target for activation of breathing in tetraplegic patients. These newest findings are being prepared for publication in peer-review scientific journals and presentation at the upcoming annual meeting for the Society for Neuroscience.
- Medicine and Medical Research
- Anatomy and Physiology