Systematic Identification of Genes Required for Expression of Androgen Receptor Splice Variants
Technical Report,01 Jun 2015,31 May 2016
University of Mississippi Medical Center Jackson United States
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Recently, a new genetic engineering tool has been developed from bacterial Clustered Regularly Interspaced Short Palindromic Repeats CRISPRCRISPR-Associated System Cas. It has several advantages over the existing ones and one of them is the ability to make a very large number tenth of thousands of gene knockouts so that we can screen genes important to prostate cancer. For example, androgen receptor AR is well known for its role in prostate cancer progression and castration resistance, and several AR splicing variants have been identified in castration resistant prostate cancer. With this technology, we can answer questions like how prostate cancer becomes castration resistant after anti-hormone therapy. Furthermore, these genes screened out with this technology may serve biomarkers or therapeutic targets. Thus, the overall of goal of this application is to identify genes involved in regulation of AR splice variant through CRISPRCas screening system.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research