A Novel Field-Deployable Point-of-Care Diagnostic Test for Cutaneous Leishmaniasis
Technical Report,30 Sep 2015,29 Sep 2016
University of Texas Medical Branch Galveston United States
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Leishmaniasis is caused by the protozoan Leishmania and is generally transmitted by the bite of sand flies of the genus Lutzomyia or Phlebotomus, The disease has significant global impact, producing 10-20 million cases of leishmaniasis worldwide. Cutaneous leishmaniasis CL is characterized by chronic skin ulcers that can impact the individuals functional status, lead to expensive and untimely treatment, and result in disfiguring scarring. Military training and combat operations resulted in cases of CL in soldiers USA, UK deployed to Central America. More recently 2003-2004, CL was reported in almost 1,200 members of the U.S. Armed Forces deployed to Iraq and Afghanistan, and the infection is an ongoing concern in the OEFOIF veteran population. To date, there is no field-standardized molecular method based on sensitive DNA amplification coupled with Lateral Flow reading to detect leishmaniasis. Isothermal amplification by RPA Recombinase Polymerase Amplification is a novel strategy to diagnose infectious diseases that can be used at the POC because it is highly sensitive, fast, inexpensive and able to work at most ambient temperatures.
- Medicine and Medical Research