Regulation of Heat Stress by HSF1 and GR
Technical Report,15 Aug 2015,14 Aug 2016
Henry M. Jackson Foundation for the Advancement of Military Medicine Bethesda United States
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The purpose of this project is to examine how activation of two cellular defense mechanisms involving heat shock transcription factor 1 HSF1 and glucocorticoid receptor GR is associated with heat tolerance and heat acclimation. This relationship will be examined in cultured mouse skeletal muscle cells task 1 in heat-tolerant versus -intolerant mice task 2 and in heat acclimated and unacclimatized mice task 3. We have completed all the cell-based tests and performed molecular assays for task 1. This work has resulted in one manuscript accepted. We have found that heat acclimation and moderate heat shock appear to have different effects on the mitochondrial morphology and fission protein in skeletal muscle cells. The signaling pathways involving HSF1 and GR are known to regulate mitochondrial function. Thus these findings suggest that mitochondrial dynamics may also serve as potential biomarkers indicating heat resistance and HA effect. In addition, in this second annual reporting period, we conducted animal experiments for tasks 2 and 3. The work of the project is progressing as projected.
- Stress Physiology