Microbial Biosensor for the Detection of Protease-Virulent Factors from Pathogens
Technical Report,26 Jun 2014,01 Nov 2016
NATIONAL UNIVERSITY OF SINGAPORE SINGAPORE Singapore
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The PI has been successful in the detection of protease-virulent factors from pathogens through whole cell and protein-based biosensor designs. Other means of detecting pathogens are reported through the detection of microbial signaling molecules and environmental cue sensing. The PI designed a protease sensing whole-cell biosensor, and they compiled a library of tools used to engineer the microbial cell to respond at different threshold concentrations and response time. This confers the advantage of flexibility in detecting the protease-toxin which can pave the road for engineering microbes to facilitate bioremediation of contaminated water sources. The protein-based biosensor created functions as a quick method of detection that offers rapid response time. Used in tandem, both of these biosensors would function to detect the presence and clear the toxin. These biosensors demonstrated could be added on to the toolkit for detection of other contaminants that would be present in the water supply. As a whole, the design of these biosensors can provide rapid identification of various types of contaminants in a water supply and ultimately serve to reduce the possibility of water-related infections for deployed individuals.