Integration of Peptides into Organic Thin Film Transistor (OTFT)-based Printable Sensors
Technical Report,22 Apr 2015,21 Oct 2016
UNIVERSITY OF NEWCASTLE RESEARCH SERVICES GRANTS OFFICE CALLAGHAN Australia
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Biosensors based on organic thin film transistor technology offer the exciting prospect of detecting and identifying peptides biomarkers found in bodily fluids. This development would assist in the monitoring or identification of medical diseases and facilitate rapid, point-of-care testing. In particular, neuropeptide Y is a highly abundant neurotransmitter with links to many important physiological functions, including stress response. Its rapid detection provides an opportunity to identify anxiety and stress related disorders such as post-traumatic stress disorder. Moreover, inkjet printing of organic electronic inks may provide a pathway for reliable, large scale production of low cost sensors. In this project, multiple detection mechanisms for two different peptides, L-reduced glutathione and neuropeptide Y, are presented. A pathway for impedance-based peptide detection using Nafion films was also presented. Receptor elements for glutathione detection were incorporated into the sensor device structure and a current response three times larger than that of water was obtained when 2 mM glutathione was placed on the sensor. This response demonstrated an increase in sensitivity of five times over the non-transistor based sensing mechanism. Electrochemical detection of neuropeptide Y was also achieved, with the method proposed, utilising monoclonal anti-neuropeptide Y antibodies, enabling detection of neuropeptide Y concentrations below 95 nM.
- Biomedical Instrumentation and Bioengineering
- Electrical and Electronic Equipment