Accession Number:

AD1031198

Title:

Generation and Characterization of Protective Antibodies to Marburg Virus

Descriptive Note:

Journal Article

Corporate Author:

USAMRIID Frederick United States

Personal Author(s):

• Froude,Jeffrey II W.
• Pelat,T.
• Miethe,S.
• Zak,S. E.
• Wec,A. Z.
• Chandran,K.
• Brannan,J. M.
• Bakken,R. R.
• Hust,M.
• Thullier,P.
• Dye,J. M.

Report Date:

2017-04-03

Pagination or Media Count:

22.0

Abstract:

Marburg virus MARV and Ebola virus EBOV have been a source of epidemics and outbreaks for several decades. We present here the generation and characterization of the first protective antibodies specific for wild-type MARV. Non-human primates NHP, cynomolgus macaques, were immunized with viral-replicon particles expressing the glycoproteins GP of MARV Ci67 isolate. An antibody fragment single chain variable fragment, scFv phage display library was built after four immunogen injections, and screened against the GP1-649 of MARV. Sequencing of 192 selected clones identified 18 clones with distinct VH and VL sequences.

Descriptors:

• viral hemorrhagic fevers
• virus diseases
• therapeutics
• antibodies
• vaccines
• health care

Subject Categories:

• Microbiology
• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE