Accession Number:

AD1030317

Title:

Development of Purification Protocol Specific for Bacteriocin 105B

Descriptive Note:

Technical Report,01 Nov 2013,31 Aug 2014

Corporate Author:

ARMY NATICK SOLDIER RESEARCH DEVELOPMENT AND ENGINEERING CENTER MA NATICK United States

Report Date:

2017-02-09

Pagination or Media Count:

20.0

Abstract:

This report summarizes work conducted to establish a purification procedure specific to a narrow-spectrum antimicrobial peptide. This antimicrobial agent, termed a bacteriocin, was evaluated in previous work and determined to exhibit activity against a pathogenic organism of interest to the Army, Bacillus anthracis Sterne, a surrogate of the active form of Bacillus anthracis. As the current application of broad-spectrum antimicrobials promotes the development of multi-drug resistant microorganisms, targeted antimicrobials are being investigated as a suitable alternative. Although bacteriocins have exhibited great promise as narrow-spectrum antimicrobial agents, their wide-spread adaptation has been limited in part due to the time-consuming and costly processes employed for development. The work cited in this report presents a robust purification procedure for an individual bacteriocin to maximize the yield of the antimicrobial peptide for employment in future assays and development into a platform, such as a textile, that relays antimicrobial activity.

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE