Characterization and Expression of Drug Resistance Genes in MDROs Originating from Combat Wound Infections
Technical Report,13 Sep 2012,12 Jun 2016
J Craig Venter Institute Rockville United States
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Twenty-five multi-drug resistant organisms MDROs of high military importance were sequenced using the Illumina HiSeq platform to identify drug resistance and virulence mechanism contributing to their success as pathogens in the wound environment. A novel bioinformatics pipeline was used to incorporate genomic sequencing data obtained from multiple sequencing platforms with optical restriction map data to assemble the genomes into sequence contigs. Bioinformatics processes for the automated identification and classification of antibioticresistance mechanisms and IS elements were developed to enable mapping of these features onto the genomes of the 25 selected MDROs. Preliminary investigations of IS element content showed considerable diversity and abundance levels across a collection of Acinetobactergenomes.