Development of Biomarkers for Assessing In Situ RDX Biodegradation Potential
Technical Report,01 Feb 2012,31 Aug 2016
Michigan State University East Lansing United States
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The objective was to identify the microorganisms and genes responsible for the biodegradation of RDX hexahydro-1,3,5-trinitro-1,3,5-triazine in mixed microbial communities using stable isotope probing, quantitative PCR and high throughput sequencing. The research targeted RDX biodegradation in mixed community samples, as these microbial communities are more representative of those at contaminated sites. The research involved four major projects, the first of which was a literature review. The second task examined the RDX degrading communities in four different soil slurries. The third task examined the microorganisms involved in RDX biodegradation from surface soils from a detonation area at a Navy Base. The fourth task investigated the microorganisms and functional genes xenA, xenB and xplA linked to RDX biodegradation at two Navy sites. Several key microorganisms were associated with RDX removal in these mixed communities. These phylogenetic and functional biomarkers will be useful to determine the biological potential for RDX degradation across sites.
- Soil Mechanics