Accession Number:

AD1024764

Title:

Targeting Histone Abnormality in Triple Negative Breast Cancer

Descriptive Note:

Technical Report,01 Aug 2015,31 Jul 2016

Corporate Author:

University of Pittsburgh Pittsburgh United States

Personal Author(s):

Report Date:

2016-08-01

Pagination or Media Count:

33.0

Abstract:

In this funding period, we continued to test the hypothesis that silencing of key tumor suppressive genes by enhanced crosstalk between LSD1 and HDAC5 is a unique epigenetic mechanism promoting TNBC growth, and blockade of the HDAC5-LSD1 axis results in profound inhibition of TNBC growth and metastasis. By using In vitro pull-down assays with His-tag recombinant LSD1 protein incubating withHDAC5 full-length or deletion mutants and immunofluorescence assays, we identified that HDAC5 domain containing nuclear localization element is essential for interaction with LSD1.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE