Targeting Histone Abnormality in Triple Negative Breast Cancer
Technical Report,01 Aug 2015,31 Jul 2016
University of Pittsburgh Pittsburgh United States
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In this funding period, we continued to test the hypothesis that silencing of key tumor suppressive genes by enhanced crosstalk between LSD1 and HDAC5 is a unique epigenetic mechanism promoting TNBC growth, and blockade of the HDAC5-LSD1 axis results in profound inhibition of TNBC growth and metastasis. By using In vitro pull-down assays with His-tag recombinant LSD1 protein incubating withHDAC5 full-length or deletion mutants and immunofluorescence assays, we identified that HDAC5 domain containing nuclear localization element is essential for interaction with LSD1.
- Medicine and Medical Research