Dehydration as a Cause of Chronic Kidney Disease: Role of Fructokinase
Technical Report,30 Sep 2015,29 Sep 2016
Regents of the University of Colorado Aurora United States
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Our studies evaluate how recurrent dehydration can cause chronic kidney disease, an important question for the military and public. We hypothesize, based on preliminary data, that dehydration associated renal injury results from hyperosmolarity induced activation of renal aldose reductase-fructokinase. We made excellent progress this last year. First, we now have the floxed KHK KO mouse and have generated successful litters so we can proceed with selective renal knockout of fructokinase in dehydration induced kidney disease. Aim 2 investigates the role of vasopressin receptors and uric acid, and we have completed studies with the Vasopressin 2 receptor submitted and have completed the experiments with V1a and V1b knockout. We have also performed some hypothalamic explant studies and documented a functional fructokinase system in the hypothalamus manuscript submitted. Aim 3 tests the role of rehydration with fructose solutions with or without blocking of vasopressin receptors. The administrative delay with our collaborators has been resolved and experiments are ongoing. In summary, we are proceeding with how recurrent dehydration causes chronic kidney disease via vasopressin and fructokinase and remain on target for finishing in time.
- Medicine and Medical Research