Monitoring of Viral Induced Cell Death Using Real Time Cell Analysis
Defence Science and Technology Group Fishermans Bend VIC Australia
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The TCID50 is a measure of infectious viral titre in a sample. Traditionally this assay has been carried out using a microscopy based assay. This assay is highly subjective and relies on the correct identification of cytopathic effect CPE by eye. In order to ascertain whether the xCELLigence real-time cell analysis RTCA platform could be used to deliver a more objective and robust measure of TCID50, a comparative study of the two methods was carried out. Two viruses were analysed, Gan Gan and Holmes Jungle viruses, members of family Bunyaviridae and Rhabdoviridae respectively. The RTCA method gave results very close to the traditional method and was significantly quicker and easier to analyse. The RTCA method also has the potential to provide other information about the viral infection of cells which if explored further could be useful.
- Medicine and Medical Research