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Kinetic Self-Assembly of DNA Tiles and Bricks

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Technical Report,01 Jun 2013,31 May 2016

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President and Fellows of Harvard College Cambridgem United States

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The objective of our work is to engineer synthetic developmental self-assembly, where a synthetic molecular structure grows isothermally in a kinetically controlled fashion. We combine the structural insight gleaned from building complex structures from single stranded DNA bricks with the kinetic insight on programming assembly pathway using hairpin motifs and strand displacement interactions. This paradigm is fundamentally different from and conceptually more powerful than the dominant thermal annealing paradigm for assembling synthetic molecular structures. We are using this developmental self-assembly framework for triggered isothermal self-assembly of highly complex single-stranded tile and bricks based nanostructures. The successful implementation of this developmental self-assembly framework will introduce a profound paradigm shift in the field of synthetic self-assembly, and promises numerous technological applications with transformative impacts. As a parallel and supplemental effort we develop software tools to automate the design and in silico testing of these structures.

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology
  • Test Facilities, Equipment and Methods

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