Treatment of Partial Thickness Burns with a Novel Extracellular Matrix in Rats (Rattus norvegicus)
60 MDG CIF Travis AFB United States
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Objectives The objective this study was to examine the cellular and immune responses to various extracellular matrices ECM in a rat burn model. The primary outcome measure was wound area remaining after four weeks. Methods Fifty male Sprague-Dawley rats were anesthetized. A 2-cm diameter partial thickness burn was produced using a brass scale weight. Groups of 10 rats were randomly assigned to the various treatments. Jackets made from bandaging material were then applied to protect the wound and rats were housed on sterile bedding. The rats were examined and dressings changed at weekly intervals for four weeks. Digital images of the wounds were taken, and tissues were harvested for histopathology. Results There was no significant difference between treatments in terms of mean wound area p 0.77. Histologic examination revealed that all of the grafts were infected, with bacteria and polymorphonucleocyte infiltration. Conclusions None of the extracellular matrices performed differently than the control treatment. However, infections likely interfered with wound healing and prevented assessment of graft infiltration. A different animal model will be needed to provide a complete evaluation of the cellular and immune response to these materials.
- Medicine and Medical Research