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Treatment of Chronic Myocardial Infarction in a Pig (Sus scrofa) Model with Extracellular Matrix and Stem Cells

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Technical Report,04 Sep 2014,13 Aug 2015

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60th Medical Group (AMC) Travis AFB United States

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Objectives The goal of this protocol was to create myocardial infarctions in mini-pigs using polystyrene microspheres to infarct a portion of the left ventricle myocardium tor future regenerative medicine studies. Methods Castrated male Yucatan mini-pigs were pre-medicated with oral amiodarone, aspirin, and clopidogrel according to protocol. Once the pigs were anesthetized a baseline echocardiogram was obtained. Under fluoroscopic guidance, a hockey stick catheter was placed in a femoral artery Into the left anterior descending LAD coronary artery. A guide wire was then placed in the first or second diagonal branch of the LAD, over which a coronary artery balloon catheter was advanced. Once in place, the balloon catheter was inflated and polystyrene microspheres were injected to occlude the vessel. The echocardiogram was repeated, and the pigs were recovered. Two weeks later, provided there were no postoperative complications, the pigs were transferred to UC Davis for maintenance and further surgery. Results Forty-nine underwent myocardial infarctions without misadventure. Infusion of polystyrene beads into a diagonal branch of the LAD resulted In a repeatable and controlled myocardial Infarction. Conclusion The method reported here provided consistent and repeatable myocardial infarcts with minimal morbidity.

Subject Categories:

  • Medicine and Medical Research
  • Anatomy and Physiology
  • Anatomy and Physiology

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