Porcine (Sus scrofa) Chronic Myocardial Infarction Model Development
Technical Report,27 Jun 2012,19 Feb 2015
60 MDG CIF Travis AFB United States
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Objectives This study was aimed at the development of a novel closed-chest porcine model of ischemic cardiomyopathy, which closely mimics the native disease found in people, and could be chronically monitored via standard transthoracic echocardiography TTE, while attempting to reduce the number of adverse events associated with alternate models. Methods Eight Yucatan miniature pigs underwent percutaneous embolization of the D1-LAD via injection of 90 microns polystyrene micro-spheres. Cardiac structure and function were monitored using TTE. Post-mortem histopathology and biochemical analysis were performed to evaluate for changes in myocardial structure and extracellular matrix composition respectively. Results Overall procedural survival rate was 83 percent 56 with one pig excluded due to failure of infarction and another due to deviation from protocol. Systolic dysfunction was appreciated following infarction denoted by a significant reduction in ejection fraction and increase in ventricular internal diameter in systole LVIDs. Histopathology showed the presence of disorganized fibrosis, and biochemical analysis was consistent with fibrotic replacement of normal myocardium. Conclusion Systolic dysfunction and changes in extracellular matrix composition induced via embolization of the D1-LAD closely mimic those found in individuals with chronic ICM, and represent a location visible without the need for anesthesia.
- Medicine and Medical Research