In Situ Bioremediation of 1,4-Dioxane by Methane Oxidizing Bacteria in Coupled Anaerobic-Aerobic Zones
Technical Report,01 Jun 2013,01 Feb 2016
CB and I Federal Service, LLC. Lawrenceville United States
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Microcosm tests, microbial enrichments, pure culture studies, and column experiments were performed to evaluate the co-metabolic biodegradation of 1,4-dioxane 1,4-D by methane-, ethane- and propane-oxidizing bacteria. Results obtained during this limited scope study showed that methane-oxidizing bacteria were not effective for promoting the biodegradation of 1,4-D. For all conditions examined, including use of copper chelators, various nutrients, pure and enrichment cultures, and soil collected from 3 different sites, 1,4-D biodegradation was not observed with methane as a cosubstrate. However, ethane- and propane-oxidizing bacteria were shown to biodegrade 1,4-D. Michaelis-Menten kinetic parameters were determined for both 1,4-D and ethane during degradation by an enrichment culture. Regression of the model parameters Vmax and Km for 1,4-dioxane were 4.6 - 1.7 x 10-5 mgmgcellhand 0.23 - 0.07 mgL, respectively Vmax and Km for ethane were 1.8 - 0.6 x 10-3 mgmgcellhand 0.064 - 0.02 mgL, respectively. Overall, the data suggest that ethane and propane are more effective cosubstrates than methane for stimulating 1,4-D biodegradation.
- Solid Wastes and Pollution and Control