A Model for Understanding the Genetic Basis for Disparity in Prostate Cancer Risk
Technical Report,21 Sep 2015,20 Sep 2016
University of Wisconsin Madison United States
Pagination or Media Count:
Prostate cancer is the most commonly diagnosed cancer in men. Among African American men, the incidence of prostate cancer is approximately 60 higher and the mortality rate in this population is 2 to 3 times greater compared with European American men. The reasons for this disparity are not completely understood. Current tools in hand to study these differences, such as genetically altered mouse models, are useful for dissecting roles of specific genes and signaling pathways in intact animal, but have limited utility for understanding differences in disease susceptibility in humans. The overall objective of this application is to model prostate epithelial cells to understand the molecular basis for the disparities in prostate cancer risk between white Caucasian and black African-American men. The specific aims are 1 to establish conditions that promote differentiation of human neonatal foreskin skin fibroblast-derived iPSC into cells with characteristics of prostate epithelium, 2 identify differences in gene expression and epigenetic signatures between prostate epithelial cells derived from iPSC of Caucasian and African-American foreskin fibroblasts and 3 compare and establish methods to transform differentiated prostate epithelial cells to identify differences in susceptibility to transformation.