Accession Number:

AD1022004

Title:

Novel Listeria Vectors Secreting Gut Flora Altering Agents to Prevent Colon Cancer and Treat Colitis

Descriptive Note:

Technical Report,15 Aug 2015,14 Aug 2016

Corporate Author:

University of Texas health Science Center at San Antonio San Antonio United States

Personal Author(s):

Report Date:

2016-09-01

Pagination or Media Count:

8.0

Abstract:

In the prior period we obtained needed IACUC and biosafety approvals, and developed the backbone Listeria vector needed to produce the B7-H1 scFv and B7-H1-expressing Listeria vectors for follow up work. We demonstrated the significance of host B7-H1 in preventingmitigating colitis and also colon cancer, suggesting that a B7-H1-expressing vector could be useful for clinical uses in colitis and cancer prevention. We generating a full PD-L1 KO cell line using CRISPR to optimize library screening for anti-B7-H1 scFv. We collected useful data on immune and signaling events in colon during DSS-induced colitis to guide studies of colon tissue explants.

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE