Novel Listeria Vectors Secreting Gut Flora Altering Agents to Prevent Colon Cancer and Treat Colitis
Technical Report,15 Aug 2015,14 Aug 2016
University of Texas health Science Center at San Antonio San Antonio United States
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In the prior period we obtained needed IACUC and biosafety approvals, and developed the backbone Listeria vector needed to produce the B7-H1 scFv and B7-H1-expressing Listeria vectors for follow up work. We demonstrated the significance of host B7-H1 in preventingmitigating colitis and also colon cancer, suggesting that a B7-H1-expressing vector could be useful for clinical uses in colitis and cancer prevention. We generating a full PD-L1 KO cell line using CRISPR to optimize library screening for anti-B7-H1 scFv. We collected useful data on immune and signaling events in colon during DSS-induced colitis to guide studies of colon tissue explants.