Accession Number:

AD1021999

Title:

nsPEF-induced PIP2 Depletion, PLC Activity and Actin Cytoskeletal Cortex Remodeling Are Responsible for Post-exposure Cellular Swelling and Blebbing

Descriptive Note:

Journal Article - Open Access

Corporate Author:

General Dynamics Information Technology JBSA Fort Sam Houston United States

Report Date:

2016-11-11

Pagination or Media Count:

7.0

Abstract:

Cell swelling and blebbing has been commonly observed following nanosecond pulsed electric field nsPEF exposure. The hypothesized origin of these effects is nanoporation of the plasma membrane PM followed by transmembrane diffusion of extracellular fluid and disassembly of cortical actin structures. This investigation will provide evidence that shows passive movement of fluid into the cell through nanopores and increase of intracellular osmotic pressure are not solely responsible for this observed phenomena. We demonstrate that phosphatidylinositol-4,5-bisphosphate PIP2 depletion and hydrolysis are critical steps in the chain reaction leading to cellular blebbing and swelling.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE