nsPEF-induced PIP2 Depletion, PLC Activity and Actin Cytoskeletal Cortex Remodeling Are Responsible for Post-exposure Cellular Swelling and Blebbing
Journal Article - Open Access
General Dynamics Information Technology JBSA Fort Sam Houston United States
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Cell swelling and blebbing has been commonly observed following nanosecond pulsed electric field nsPEF exposure. The hypothesized origin of these effects is nanoporation of the plasma membrane PM followed by transmembrane diffusion of extracellular fluid and disassembly of cortical actin structures. This investigation will provide evidence that shows passive movement of fluid into the cell through nanopores and increase of intracellular osmotic pressure are not solely responsible for this observed phenomena. We demonstrate that phosphatidylinositol-4,5-bisphosphate PIP2 depletion and hydrolysis are critical steps in the chain reaction leading to cellular blebbing and swelling.
- Medicine and Medical Research