Electrokinetic enrichment and detection of neuropeptide for performance monitoring
Technical Report,03 Jun 2014,02 Dec 2015
VIRGINIA UNIV CHARLOTTESVILLE CHARLOTTESVILLE United States
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The real-time monitoring of human performance biomarkers for fatigue, vigilance, and stress among DoD personnel during key missions requires devices that enable facile detection of multiple targets with minimal user intervention. Since the biomarkers are present at dilute levels, there is a need for biomarker enrichment under electrical and magnetic force fields within microfluidic devices, so that each relevant target may be detected versus high levels of interfering molecules within biofluids. The optimized conditions of these force fields can then be routinely applied within field settings for facile electrical and optical detection, with minimal user intervention. In this project, we developed nano-slit devices and optimized the electrokinetic preconcentration conditions for key neurological biomarkers of interest, by using nanoparticles and aptamers to enhance specificity. Additionally, biomarker preconcentration was coupled to various detection paradigms to achieve high-sensitivity biomarker profiles for future application towards unraveling the signaling pathways for assessing and mitigating stress.
- Electricity and Magnetism
- Anatomy and Physiology