Factors Essential for Prostate Cancer Metastasis Revealed Through a Novel 3D Microtissue Assay
Technical Report,01 Apr 2015,31 Mar 2016
Case Western Reserve University Cleveland United States
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Growing evidence suggest that the micro-dissemination of bone metastatic prostate cancer BMPCa to bone marrow BM may be facilitated in early-stage of cancer development and could be the origin of future outgrowth and metastasis. Importantly, the osteoblastic niche is known to constitute the sanctuary for bone-homing cancer cells where it confers BMPCa dormancy and therapy-resistance. Under the support of current proposal, we have carefully validated a novel 3D osteoblastic niche 3D-ObN assay system which recapitulate BMPCa and osteoblastic niche interactions. Throughout the research year we have made following progressions.1 We verified that the 3D-ObN can recruit BMPCa as well as other metastatic cancers.2 We conducted a novel application of Hypoxia-sensitive reporters to measure BMPCa invasion ability and to apply for HTS.3 3D-ObN recruits cancer cells depending on high metastatic ability, cancer stem-like subsets, and CXCR4-CXCL12 interaction.