Accession Number:

AD1016881

Title:

Factors Essential for Prostate Cancer Metastasis Revealed Through a Novel 3D Microtissue Assay

Descriptive Note:

Technical Report,01 Apr 2015,31 Mar 2016

Corporate Author:

Case Western Reserve University Cleveland United States

Personal Author(s):

• Lim,Seunghwan

Report Date:

2016-06-01

Pagination or Media Count:

9.0

Abstract:

Growing evidence suggest that the micro-dissemination of bone metastatic prostate cancer BMPCa to bone marrow BM may be facilitated in early-stage of cancer development and could be the origin of future outgrowth and metastasis. Importantly, the osteoblastic niche is known to constitute the sanctuary for bone-homing cancer cells where it confers BMPCa dormancy and therapy-resistance. Under the support of current proposal, we have carefully validated a novel 3D osteoblastic niche 3D-ObN assay system which recapitulate BMPCa and osteoblastic niche interactions. Throughout the research year we have made following progressions.1 We verified that the 3D-ObN can recruit BMPCa as well as other metastatic cancers.2 We conducted a novel application of Hypoxia-sensitive reporters to measure BMPCa invasion ability and to apply for HTS.3 3D-ObN recruits cancer cells depending on high metastatic ability, cancer stem-like subsets, and CXCR4-CXCL12 interaction.

Descriptors:

• prostate cancer
• metastasis
• CELLULAR MICROENVIRONMENT
• osteoblasts
• stem cells
• bone marrow

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE