A Genome-Wide Knockout Screen to Identify Genes Involved in Acquired Carboplatin Resistance
Technical Report,15 Jun 2015,14 Jun 2016
University of California, San Diego La Jolla United States
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The specific aims of this project are to 1 Perform a GeCKO library screen to identify genes that when knocked out render human ovarian cells 2.5-fold resistant to CBDCA 2 Validate the ability of candidate gens to control sensitivity to CBCDA in vitro and in vivo, and 3 Functionally annotate the candidate genes using public resources. Substantial progress has been made on specific aim 1. This progress report covers work done from June 15, 2015 through May 31, 2016. Significant achievements to date include the generation of three Cas9- expressing ovarian cell lines the generation of CBDCA cytotoxicity killing curves in three ovarian cell lines the production of GeCKO v2.0 lentivirus and, tittering of virus against HEK293 cells and a panel of human ovarian carcinoma cell lines.