Accession Number:

AD1016117

Title:

Electroporation of Mammalian Cells by Nanosecond Electric Field Oscillations and its Inhibition by the Electric Field Reversal

Descriptive Note:

Journal Article - Open Access

Corporate Author:

711th Human Performance Wing, Air Force Research Laboratory JBSA Fort Sam Houston United States

Report Date:

2015-09-08

Pagination or Media Count:

11.0

Abstract:

The study compared electroporation efficiency of bipolar and unipolar nanosecond electric field oscillations NEFO. Bipolar NEFO was a damped sine wave with 140 microsecs first phase duration at 50 height the peak amplitude of phases 2-4 decreased to 35 ,12, and 7 of the first phase. This waveform was rectified to produce unipolar NEFO by cutting off phases 2 and 4. Membrane permeabilization was quantified in CHO and GH3 cells by uptake of a membrane integrity marker dye YO-PRO-1 YP and by the membrane conductance increase measured by patch clamp. For treatments with 1-20 unipolar NEFO, at 9.6-24 kVcm, 10 Hz, the rate and amount of YP uptake were consistently 2-3-fold higher than after bipolar NEFO treatments, despite delivering less energy. However, the threshold amplitude was about 7 kVcm for both NEFO waveforms. A single 14.4 kVcm unipolar NEFO caused a 1.5-2 times greater increase in membrane conductance p0.05 than bipolar NEFO, along with a longer and less frequent recovery. The lower efficiency of bipolar NEFO was preserved in Ca2 -free conditions and thus cannot be explained by the reversal of electrophoretic flows of Ca2. Instead, the data indicate that the electric field polarity reversals reduced the pore yield.

Subject Categories:

  • Electrical and Electronic Equipment

Distribution Statement:

APPROVED FOR PUBLIC RELEASE