Accession Number:
AD1016117
Title:
Electroporation of Mammalian Cells by Nanosecond Electric Field Oscillations and its Inhibition by the Electric Field Reversal
Descriptive Note:
Journal Article - Open Access
Corporate Author:
711th Human Performance Wing, Air Force Research Laboratory JBSA Fort Sam Houston United States
Personal Author(s):
Report Date:
2015-09-08
Pagination or Media Count:
11.0
Abstract:
The study compared electroporation efficiency of bipolar and unipolar nanosecond electric field oscillations NEFO. Bipolar NEFO was a damped sine wave with 140 microsecs first phase duration at 50 height the peak amplitude of phases 2-4 decreased to 35 ,12, and 7 of the first phase. This waveform was rectified to produce unipolar NEFO by cutting off phases 2 and 4. Membrane permeabilization was quantified in CHO and GH3 cells by uptake of a membrane integrity marker dye YO-PRO-1 YP and by the membrane conductance increase measured by patch clamp. For treatments with 1-20 unipolar NEFO, at 9.6-24 kVcm, 10 Hz, the rate and amount of YP uptake were consistently 2-3-fold higher than after bipolar NEFO treatments, despite delivering less energy. However, the threshold amplitude was about 7 kVcm for both NEFO waveforms. A single 14.4 kVcm unipolar NEFO caused a 1.5-2 times greater increase in membrane conductance p0.05 than bipolar NEFO, along with a longer and less frequent recovery. The lower efficiency of bipolar NEFO was preserved in Ca2 -free conditions and thus cannot be explained by the reversal of electrophoretic flows of Ca2. Instead, the data indicate that the electric field polarity reversals reduced the pore yield.
Descriptors:
Subject Categories:
- Electrical and Electronic Equipment