Pathology Report for One-Generation Study of the Effects of 3-nitro-1,2,4-triazol-5-one (NTO) to the Northern Leopard Frog (lithobates pipiens) Under Static Renewal Test Conditions
Technical Report,01 Oct 2015,29 Feb 2016
Army Public Health Center (Provisional) Aberdeen Proving Ground United States
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Larval tadpole northern leopard frogs were exposed to 3-nitro-1,2,4-triazol-5-one NTO or to water control until they developed into froglets up to 90 days at Gosner Stage 46 Gosner, 1960. Animals were humanely euthanized and kidney and liver tissues examined microscopically to detect indications of toxicity. No evidence of toxicity or developmental delay was observed, suggesting NTO may not affect anuran renal or hepatic development. Tissues did have inflammatory changes normally associated with infection by mycobacteria, fungi or foreign materials.