Accession Number:

AD1014064

Title:

Repair of Neocortex in a Model of Cortical Dysplasia

Descriptive Note:

Technical Report

Corporate Author:

Uniformed Services University Of The Health Sciences Bethesda United States

Personal Author(s):

Report Date:

2007-03-27

Pagination or Media Count:

151.0

Abstract:

Our lab developed an animal model to elucidate factors associated with abnormal neocortical development and to attempt repair of cortical dysgenesis. We disrupt corticogenesis using an anti-mitotic methylazoxy methanol MAM, which inhibits mitosis for several hours. The effects of MAM on neocortical development are assessed during early embryonic day 24 E24 and late E33 corticogenesis in ferrets. These animals have protracted cortical development with neurogenesis and migration continuing postnatally. MAM treatment on E24 leads to disorganized cortical laminae, abnormal radial morphology, precocious differentiation of radial glia, and dispersal of Cajal Retzius cells. MAM treatment on E33 leads to less severe effects including diminished layer 4, widespread termination of thalamocortical afferents, and abnormal distribution of GABAA-alpha receptors.

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE