During instrumentation of a root canal, ethylenediaminetetraacetic acid EDTA as a chelating agents may be used during cleaning and shaping. EDTA demineralizes dentin and may inadvertently contribute to root canal transportation. The purpose of this study was to evaluate the effect of chelating agents used in moderately curved canals during instrumentation using micro-computed tomography microCT and imagingmodeling technology. Methods Moderately curved mesiobuccal root canals of 18 de-identified maxillary molars were standardized in length and divided into 3 groups n6. Initial scans were taken using microCT . All canals were instrumented with Profile DENTSPLY Tulsa Dental, Tulsa, OK 0.04 taper NiTi rotary instruments using saline, 17 EDTA Pulpdent Corp., Watertown, MA or RC-Prep Premier Dental, Philadelphia, PA, USA. After instrumentation, lengths were re-measured and all the teeth were re-scanned. Using computer software, 3-dimensional models were created from the pre and post-instrumentation scans. Models were compared for changes in volume and transportation. Results There was no statistically significant difference between the groups for any of the variables evaluated working length p0.562, volume p0.079 and transportation p0.167 to 0.776. Conclusion Under the conditions of this study, the use of chelating agents during instrumentation did not affect the size or position of the root canals.