Accession Number:

AD1013095

Title:

Defining Key Entry Events for Crimean-Congo Hemorrhagic Fever Virus in Mammalian Cells

Descriptive Note:

Technical Report

Corporate Author:

Uniformed Services University Of The Health Sciences Bethesda United States

Personal Author(s):

• Garrison,Aura R.

Report Date:

2012-08-10

Pagination or Media Count:

147.0

Abstract:

Crimean Congo hemorrhagic fever virus CCHFV is an arthropod-borne virus in the genus Nairovirus within the family Bunyaviridae that causes significant morbidity and mortality in humans. Little is known about CCHFV-host cell interactions, in part due to the requirement for Biological Safety Level 4 containment for studies using infectious virus. Entry studies on other members of the family Bunyaviridae indicate that most of the viruses enter by clathrin-mediated endocytosis CME. However, a clathrin-independent mechanism was described for one virus within the family, which was a cell-dependent phenomenon. All bunyaviruses that have been studied also require endosomal acidification for entry, with some entering through early endosomes EE and others through late endosomes LE.The goal of this study was to define key cellular entry events for CCHFV.

Descriptors:

• Hemorrhagic fevers
• viruses
• Arthropod borne diseases
• Tickborne viruses
• animal diseases
• Infectious disease transmission
• cell line
• cells(biology)

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE