GC-MS/MS Analyses of Biological Samples in Support of Developmental Toxic Effects on Percutaneous Exposure of Rats to VX
Technical Report,01 Apr 2013,31 Jul 2013
ARMY EDGEWOOD CHEMICAL BIOLOGICAL CENTER APG MD ABERDEEN PROVING GROUND
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This report documents the results of gas chromatography-tandem mass spectrometry GC-MSMS analyses of blood, tissues, and organs heart, lung, liver, kidney, brain, eye, diaphragm, and skin that were obtained from rats postnatal days 42 and 70 percutaneously exposed to various concentrations of O-ethyl S-2-diisopropylaminoethyl methylphosphonothioate VX. The amount of O-ethyl methylphosphonofluoridate VX-G present in these biological samples was quantified using chemical ionization mass spectrometry with isotope dilution. In addition, free VX present on the skin surface was quantified from cellophane tape strip sampling using liquid chromatography-tandem mass spectrometry LC-MSMS.