Osmotic Stress Induces Transcriptional Changes in Vasopressin and Vasopressin 1b Receptor Gene Expression
Uniformed Services University Of The Health Sciences Bethesda United States
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Arginine vasopressin AVP plays a critical role in the regulation of mammalian salt and water homeostasis. To further define central nervous system adaptation to osmotic challenges, transcription of AVP and vasopressin Ib receptor VlbR genes by magnocellular neurons of the hypothalamus and epithelial cells of the choroid plexus wasstudied using ill situ hybridization. Compared to animals given a single injection of normal saline, animals that received a single injection of hypertonic saline had increases in AVPheteronuclear RNA hnRNA after 15 and 30 minutes. Animals given an injection ofhypertonic saline followed by a second injection of hypertonic saline H-H had an increase in AVP hnRNA levels that were equivalent to the response seen after a single hypertonic saline injection. Levels of AVP hnRNA after H-H were greater than the levels detected in animals given an injection of normal saline followed by a second injection of hypertonic saline N-H. This is the first study to show repeated exposure to hypertonic saline causes an immediate, robust, and reproducible increase in vasopressin gene transcription.
- Medicine and Medical Research
- Genetic Engineering and Molecular Biology