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Regulation of 2-5A Dependent RNase at the Level of its Phosphorylation

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Technical Report

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Uniformed Services University Of The Health Sciences Bethesda United States

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The 2-5A system is an interferon-regulated, antiviral pathway which results in the degradation of viral RNA and may also be involved in cell growth control. Interferon treatment of cells induces 2-5A synthetases which, upon activation by double stranded RNA, produce 2,5-linked oligoadenylates 2-5A. The only well-characterized, biochemical effect of 2-5A is its activation of 2-5A dependent RNase, an interferon-inducible 80 kDa protein that cleaves single stranded RNA. Here I describe a novel level of regulation in the 2-5A pathway. Since 2-5A dependent RNase has been implicated in growth control, my objective was to determine, how activators of protein kinase C, which in several cell lines stimulate growth or inhibit differentiation would lead to qualitative as well as quantitative changes in the pattern of 2-5A dependent RNase. Murine JLS-V9R cells were treated with interferon in the presence or absence of activators or an inhibitor of protein kinase C or a phosphatase inhibitor.

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