Advanced Lung Cancer Screening: An Individualized Molecular Nanotechnology Approach
Technical Report,01 Aug 2012,31 Dec 2015
The Johns Hopkins University Baltimore United States
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This grant utilizes complimentary approaches to improve the early detection of lung cancer. Our goal is to explore whether detection of DNA methylation changes and enhanced CT evaluations will add to the specificity of lung cancer detection. Based on our previous development of an improved panel of genes hypermethylated in lung cancer, with extraordinarily high specificity and sensitivity, we combined the improved methods of MOB with highly sensitive methylation specific PCR assays suitable for biologic fluid testing sputum and serum and completed the study of a cohort of cancer positive and negative samples. In combination with these molecular detection approaches, we have examined the alterations in air space for improving detection of lung cancer and find that variability of air spaces is associated with the presence of lung cancer. We have during the period of this grant developed a highly sensitive and specific method for early detection of lung cancer.