Correlative Light and Electron Microscopy (CLEM) and its applications in infectious disease
USAMRIID Frederick United States
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Correlative light and electron microscopy CLEM is an effective technique used to study biological samples. Signal-specific indicators observable under light microscopy LM allow scientists to locate areas of interest for high resolution ultra-structure observation under the electron microscope EM. Recent method developments provided breakthroughs creating an effective, direct, and accurate research diagnostic tool for functionally related structural biological studies. CLEM is particularly useful in infectious disease research where there is need to study, at nanoscale, objects of interest which are commonly part of rare transient events or afflict a particular cell among a majority of unaffected cells. In this review we discuss several CLEM methods, summarize currently available fluorescent markers, and discuss CLEM instrumentation setups for novel approaches to imaging cellular events in infectious disease.