Preventing Prostate Cancer Metastasis by Targeting Exosome Secretion
Technical Report,30 Sep 2013,29 Sep 2015
New York University New York United States
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We hypothesized that exosomes secreted by advanced prostate cancer cells groom stromal cells at pre-metastatic sites to render the bone microenvironment more favorable for metastatic growth. Testing this hypothesis has direct relevance towards an understanding of the often lethal complications of prostate cancer, manifested in extensive and painful metastasis of the bone. We proposed compare the impact of exosomes derived from advanced stage prostate cancer on bone stromal cells by extracting vesicles from medium conditioned by metastatic and non-metastatic prostate cancer cells. We successfully established sensitive cancer proteomics analyses in our lab. We optimized the growth conditions and experimental parameters to test exosome secretion and effects in prostate cancer cells and bone marrow cells. We found that the cell lines in contrast to tissue samples do not secrete enough exosomes for efficient quantitative analysis, as was verified by a number of orthogonal approaches.