Development of a Microfluidic Platform to Analyze Evolution of Programmed Bacterial Death
Technical Report,01 Sep 2014,31 May 2015
Duke University Durham United States
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Evolution of programmed cell death in bacteria is a poorly understood phenomenon in biology. A critical limitation is the lack of high through put technology to examine dynamics of small bacterial populations. To address this limitation, we proposed to develop a drop let based microfluidic technology to generate population bottleneck. This platform will serve as a critical foundation for our long-term goal to develop a quantitative understanding of PAD evolution by using engineered bacteria. For the proposed pilot study, we will focus on the technology development. In particular, our proposed work consists of following specific tasks, focusing on proof-of-concept demonstration of this device and its use for analyzing engineered bacteria.