Accession Number:

AD1008618

Title:

Analysis of a Novel Microbial Ion Channel

Descriptive Note:

Technical Report,10 Jun 2010,09 Mar 2015

Corporate Author:

University of Texas at Austin Austin United States

Personal Author(s):

Report Date:

2016-02-04

Pagination or Media Count:

7.0

Abstract:

We discovered some sequences in the genomes of single celled eukaryotes that resembled incomplete ion channels. Since some of the organisms that have these sequences are responsible for carrying diseases, we wished to investigate whether these sequences coded for viable ion channels. If so, then we hoped that they would be good targets for drug development. We cloned this ion channel from a fungus and studied the channels expression by injecting it in frog eggs. We noted small currents that had unusual electrical properties but eventually concluded that these currents are artifacts produced by their stimulating an ion current found in frog eggs. We next planned to test whether these incomplete channels function, instead, as dominant negatives, assembling with and inhibiting complete channels. However, we did not have enough time to complete this aim in the allotted funding period.

Subject Categories:

  • Microbiology
  • Genetic Engineering and Molecular Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE