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Molecular Genetic Equipment for Improved Inventory and Monitoring of Species of Conservation Concern on Department of Defense Lands

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Technical Report,22 Jul 2013,21 Sep 2014

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University of Idaho Moscow United States

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The Laboratory for Ecological, Evolutionary and Conservation Genetics in the College of Natural Resources at the University of Idaho wastasked with designing methods to monitor species of concern on DoD lands as part of four DoD grants. With the funds granted we were ableto 1 upgrade our computing lab with new computers and versions of software and 2 expand the capacity of the lab for a higher throughputof samples. Areas of expanded capacity and throughput include DNA extraction and sample tracking extraction robot, centrifuges,barcoding equipment, DNA amplification and quantification standard and quantitative PCR machines, sample storage refrigerators andfreezers. This expanded capacity has allowed for the analysis of over 5000 fecal samples from terrestrial vertebrate species and over 1400environmental DNA from aquatic vertebrate species. The equipment purchases resulted in the acquisition of new skills for 19 laboratorypersonnel and the analyses of data generated from the new equipment led to seven peer reviewed publications and 19 meeting presentations.

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