Accession Number:

AD1008200

Title:

Depth-Resolved Nanospray Desorption Electrospray Ionization Mass Spectrometry in Biofilms

Descriptive Note:

Technical Report,09 Jul 2012,08 Apr 2013

Corporate Author:

Washington State University Pullman United States

Personal Author(s):

Report Date:

2015-11-16

Pagination or Media Count:

11.0

Abstract:

The goal of this project was to develop a nano-DESI sensor which can be used to obtain in situ, depth-resolved analyses of metabolites and possibly proteins from biofilms at different depth. A microcapillary system was developed and tested for this goal. The microcapillary system delivered a solvent ethanol from one capillary while the other capillary collected and transported a charged liquid to the mass spectrometer inlet. We tried different configurations of microcapillary systems and optimized follow rates and its operation. The project successfully demonstrated that a dual microcapillary system can be used to collect nanoliter sample from the biofilms at different depth. However, the microcapillary system also collected cells which failed its use for MS.

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE