Depth-Resolved Nanospray Desorption Electrospray Ionization Mass Spectrometry in Biofilms
Technical Report,09 Jul 2012,08 Apr 2013
Washington State University Pullman United States
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The goal of this project was to develop a nano-DESI sensor which can be used to obtain in situ, depth-resolved analyses of metabolites and possibly proteins from biofilms at different depth. A microcapillary system was developed and tested for this goal. The microcapillary system delivered a solvent ethanol from one capillary while the other capillary collected and transported a charged liquid to the mass spectrometer inlet. We tried different configurations of microcapillary systems and optimized follow rates and its operation. The project successfully demonstrated that a dual microcapillary system can be used to collect nanoliter sample from the biofilms at different depth. However, the microcapillary system also collected cells which failed its use for MS.