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In-Depth Analysis of Citrulline-Specific CD4 T-Cells in Rheumatoid Arthritis

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Technical Report,10 Dec 2014,09 Oct 2015

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Seattle Institute for Biomedical and Clinical Research Seattle United States

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The goal of this project is to test the hypothesis that cit-specific CD4 T cells present in rheumatoid arthritis RA patients exhibit a distinct cell surface phenotype and transcriptional signature that could be used to predict disease, response to therapy and identify novel therapeutic targets for the treatment of RA. We have accomplished our major goals for year 1. We began patient recruitment and to date have consented 70 of the subjects for the cross-sectional study Aim 1 and 40 of the subjects for the longitudinal study Aim 3. We also accomplished our goal to develop new tools for characterizing cit-specific T cells. We developed both a panel of HLA-DRB10404 tetramers, expanded our panel of HLA-DRB10401 tetramers, and developed multiplexing technology for evaluation of multiple specificities in a single assay. We also worked on optimizing the RNAseq methodology for transcriptional profiling Aim 2. We were able to obtain high quality RNAseq data from bulk sorted Tmr cells with as few as 50 cells total. Given these accomplishments in Year 1, we do not anticipate any problems in the next year and are on track to achieve our Year 2 goals

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