Nitric Oxide Generating Polymeric Coatings for Subcutaneous Glucose Sensors
Technical Report,15 Sep 2006,14 Sep 2007
University of Michigan Ann Arbor United States
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Heretofore, efforts to develop implantable sensors for real-time clinical monitoring of glucose subcutaneously SQ in diabetic patients have been hindered by the unreliable analytical results owing largely to biocompatibility problems induced by sensor implantation e.g., inflammatoryforeign body response. The goal of this research program is to explore and optimize the chemistries required to fabricate implantable amperometric glucose sensors with outer polymeric coatings that slowly generate low levels of nitric oxide NO. Release of NO has been shown to enhance the biocompatibility of the implanted sensors by decreasing the inflammatory response. The focus of this research has been to develop new polymeric coatings biomedical hydrogels and polyurethanes that possess immobilized copper ion sitesor organoselenium and organotellurium species that will serve as catalytic sites for in situ conversion of endogenous nitrosothiol species RSNO to NO, thereby providing a sustained local generation of NO species at the surface of the implanted sensors. Preliminary biocompatibility experiments suggest that RSNO levels within the SQ fluid of rats may be sufficient to generate enough local NO to reduce the inflammatory response at the implant site. New needle type sensors are being developed to determine the levels of RSNOs in the SQ region. Finally, functional needle type SQ glucose sensors have been prepared with both NO release and NO generation coatings. These sensors provide the basis of assessing if NO generationreleasing chemistries are compatible with glucose sensing chemistries.