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Diagnosis and Treatment of Heterotopic Ossification

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Technical Report,30 Sep 2014,29 Sep 2015

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Baylor College of Medicine Houston United States

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We recently developed a model of heterotopic ossification HO that suggests blocking the initial response of the nerve could ultimately block this devastating problem before it occurs. Studies proposed in this application to identify and isolate osterix cells have led us to identify a second tentative progenitor within peripheral nerves. These cells reside in the endoneurial compartment and express Claudin 5, PDGFRalpha, and osterix but are negative for the Schwann cell marker P75 and perineurial marker Claudin 1. We have isolated the Claudin 5, PDGFR and negative populations and confirmed that 100 percent of the osterix expression co-purified with the claudin 5 population. Claudin 5 is present on specialized endothelial cells within the endoneurium and is responsible for forming the tight junction of blood nerve interface. We are further characterizing these cells for expression of endothelial markers. We also looked at the expression of Wnt1, a marker of neural crest stem cells. Our preliminary data suggests that these cells undergo significant expansion after induction of HO however, they remained localized within the peripheral nerves. However, 100 percent of the Wnt1 cells appeared to be also claudin 5 suggesting that the Wnt1 cells may be precursors to a more differentiated progenitors. We are currently following up on these and other experiments proposed in the application.

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