Accession Number:

AD1007471

Title:

Engineering of an Extremely Thermostable Alpha/Beta Barrel Scaffold to Serve as a High Affinity Molecular Recognition Element for Use in Sensor Applications

Descriptive Note:

Technical Report,01 Aug 2012,31 Jul 2015

Corporate Author:

Columbia University New York United States

Personal Author(s):

Report Date:

2015-12-23

Pagination or Media Count:

16.0

Abstract:

The overall goal of the project was to evolve a highly thermostable enzyme alcohol dehydrogenase D AdhD from Pyrococcus furiosus to bind an explosive molecule, RDX. The enzyme naturally catalyzes the nicotinamide cofactor-dependent oxidation or reduction of alcohols, aldehydes, ketones and carbohydrates. Directed evolution techniques were used to convert the enzyme into a simple binder of the small molecule RDX. Novel binders have been identified and characterized with sub-millimolar dissociation constants. These could be further developed for use in robust and low cost biosensors.

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE