Subgrouping Chronic Fatigue Syndrome Patients By Genetic and Immune Profiling
Technical Report,08 Sep 2012,07 Sep 2015
The Leland Stanford Junior University Menlo Park United States
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We have successfully performed all testing of the approximately 600 samples 200 CFSME patients vs 397 healthy controls we set up to do under this grant at the Stanford Human Immune Monitoring Center HIMC and the Stanford HLA Mindrinos lab. These samples were tested by two novel methods CyTOF-phosphoflow and HLA Typing, respectively to help us better understand the roles of immune responses and genetics in the pathogenesis of chronic fatigue syndrome CFSmyalgic encephalomyelitis ME. It is likely that these findings will also result in an improved understanding of the pathogenesis of Gulf War IllnessGWI. For both methods, CyTOF-phosphoflow and HLA Typing, we have reviewed in excruciating detail all the potential sources of technical error in both methods, systematically cleaned all confounders and plate effects, and have set up a statistical plan.